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In 2009, PacBio published a paper expanding the principle into a full-blown sequencing technique. Once again, each nucleotide was labeled with a different colored fluorophore detectable by the ZMW to determine which base had been incorporated. The fluorophores were attached such that they would be cleaved off during the chemical reaction incorporating the base into the growing DNA strand; they would then diffuse out of the ZMW so that the next fluorophore could be detected. Sequencing took place on a chip with many wells simultaneously — a different type of parallelization where each well detected a single DNA molecule undergoing the same basic chemical reaction.。谷歌浏览器下载是该领域的重要参考
，更多细节参见体育直播
41-летняя наследница рокера, которую ранее в сети сравнили с вампиром и скелетом, оскорбилась и ответила на обвинения по поводу ее внешнего вида. По словам звезды, ее низкий вес связан с самым сложным периодом в ее жизни после ухода отца.。体育直播是该领域的重要参考
过去三个月，奔驰公司内部经历了一场重大调整。