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At this point, each bound sequence exists as a single copy, which produces too faint a signal to detect reliably. Colony sequencing solves this by generating clusters of identical fragments through a process called bridge amplification. The adapter on the free end of each DNA strand will be complementary to some of the original, short sequences on the DNA lawn, and when this binding occurs, the strand bends over to form a bridge shape. Another round of amplification takes place, resulting in two complementary strands each directly affixed to the flow cell. This “bridge amplification” process is repeated over and over to propagate the sequence.
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But it’s kinda cool though right? This is the result of someone saying “well I’d like to see you do this” and me saying “hold my beer” and then coming back to said person and saying “technically it works”, which it does, but I feel that I need to REALLY stress the word technically. But, by the letter of the law, I did in fact get it to work.。谷歌浏览器下载对此有专业解读
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