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Before agar, microbiologists had experimented with other foodstuffs as microbial media. They turned to substances rich in the starches, proteins, sugars, fats, and minerals that organisms need for growth, testing with broths, bread, potatoes, polenta, egg whites, coagulated blood serums, and gelatine. However, none worked particularly well: all were easily broken down by heat and microbial enzymes, and their surface, once colonized, became mushy and unsuitable for isolating microbes.

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Foundational to this approach is the need to cross from the Unreal C++ boundary into the C# DLL code. This boundary is inherently risky as it lacks much of the standard safety checks we normally rely on in managed code.